|Name||Miss Brooke Barnes|
|Organization||University of Florida|
SINGLE LAYERED VESICLES COMPOSED OF POLYPEPTIDE BLOCK COPOLYMERS FOR DRUG DELIVERY: THE ROAD TO SIZE MONODISPERSITY
Brooke E. Barnes, Lauren Stein, Craig Machado, Ian Smith, Daniel A. Savin
The George and Josephine Butler Polymer Research Laboratory, Department of Chemistry and Center for Macromolecular Science and Engineering, University of Florida, Gainesville, 32611, USA
Herein we report the synthesis and solution characterization of an ABC triblock consisting of poly(ethylene oxide-b-(leucine-s-valine)-b-lysine). It is hypothesized that this polymer will self-assemble into monolayered, size monodisperse vesicles in dilute aqueous solutions, independent of preparation methods. Poly(leucine) is a hydrophobic, alpha helix forming polypeptide that has been shown to exhibit a “zipper effect” in coiled-coil dimers. This specific interaction afforded by the poly(leucine) block will be able to dominate the thermodynamics of polymer self-assembly through side-by-side ordering of alpha helices, which helps drive vesicle formation. Through the incorporation of the poly(leucine) into the center of the triblock, we believe the leucine zipper will allow the helices to pack tighter, defining a nearly-constant interfacial curvature of the system. Since polymer self-assembly is dictated by core chain stretching, corona chain repulsions, and interfacial curvature the leucine block will dictate both the interfacial curvature and core chain stretching. We believe this will result in size monodisperse vesicles- a desirable quality for drug delivery systems.