|Name||Mr. Anthony Castellanos|
|Organization||Florida International University|
CHARACTERIZATION OF DIET-INDUCED MOLECULAR CHANGES IN OVARIAN RESERVES OF AEDES AEGYPTI USING 3D-MSI-TOF-SIMS AND MALDI FT-ICR MS
Anthony Castellanos,1 Quentin Vanbellingen,1 Paolo Benigni,1 Veronika Michalkova,2 Marcela Nouzova,2 Fernando Noriega,2 and Francisco Fernandez-Lima1,3
1Department of Chemistry and Biochemistry, Florida International University, Miami, Florida, 33199, United States
Female mosquitoes are subject to ‘trade-offs’ between the energetic demands of reproduction and the energy required to survive. The mobilization of nutrient reserves within the ovaries during previtellogenic development is vital for the reproductive viability of Aedes aegypti, and therefore, plays an important role in fundamental biology and vector control strategies. Previous studies have shown that both hormonal regulation and nutrition from sugar sources are determining factors of successful oocyte development into gametes. In the present work, new mass spectrometry imaging (MSI) tools were developed to characterize single ovarian follicles from mosquitoes raised with either a nutrient based or water diet in order determine biomarkers that may predict oocyte fate at the single cell level. When compared with traditional staining and microscopy approaches, the new MSI approaches result in superior and higher molecular coverage at the single follicle level.