|Name||Dr. Alfons Schulte|
|Organization||University of Central Florida|
MICRO-SPECTROSCOPY OF BIO-ASSEMBLIES AT THE SINGLE CELL LEVEL
Jeslin Kera1,2, Debopam Chakrabarti2, Alfons Schulte1
1Physics Department, 2Burnett School of Biomedical Sciences, University of Central Florida, Orlando
Confocal absorption microscopy has the benefits of requiring no labels and low light intensity for excitation while providing a signal from the contrast generated by the attenuation of propagating light due to absorption. This enables spatially resolved measurements of single live cells and bio-molecules in nano-liter solutions. We present experiments on red blood cells and model systems over the spectral range from the near-infrared to the ultraviolet. The spectral characterization of bio-assemblies with spatial resolution at the cellular level (1-2 microns) will be discussed.